Development and Validation of a Method for Detecting and Quantifying Mitragynine in Kratom Samples Using HPLC-PDA

Neni Isnaeni; Asep Saefumillah; Antonius Herry Cahyana

doi:10.54384/eruditio.v4i2.209

Penulis

Neni Isnaeni Indonesian Food and Drug Authority
Asep Saefumillah Faculty of Mathematics and Natural Sciences, University of Indonesia
Antonius Herry Cahyana Faculty of Mathematics and Natural Sciences, University of Indonesia

DOI:

https://doi.org/10.54384/eruditio.v4i2.209

Kata Kunci:

Kratom, Mitraginin, Mitragyna speciosa, Deteksi, Kuantifikasi, KCKT-PDA

Abstrak

Kratom (Mitragyna speciosa Korth) telah ditetapkan sebagai senyawa psikoaktif baru (NPS) oleh United Nations Office on Drugs and Crime (UNODC) dan digolongkan ke dalam daftar bahan terlarang dalam suplemen makanan dan obat tradisional oleh BPOM. Oleh karena itu, diperlukan metode analisis yang cepat, mudah, dan andal untuk mendeteksi dan menetapkan kandungan tanaman tersebut dan produk turunannya. Pada penelitian ini dikembangkan metode untuk mendeteksi dan menetapkan kadar produk kratom berdasarkan senyawa unik, mitragynine sebagai biomarker. Penelitian sebelumnya yang telah dikembangkan menggunakan GC-MS, LC-MS/MS, UPLC dan HPLC-PDA. Metode HPLC-PDA yang tersedia menggunakan kolom C8, namun untuk efisiensi, perlu juga dikembangkan metode pengujian dengan menggunakan kolom C18. Analisis dilakukan menggunakan sistem KCKT - PDA yang dilengkapi dengan kolom Waters Atlantis T3-C18 (250 x 4,6 mm, 5µm). Fase gerak terdiri dari asetonitril dan asam format 0,05%, pH 5,0 (75:25 v/v) dengan laju alir 1,0 mL/menit. Deteksi dilakukan pada panjang gelombang 225 nm. Validasi metode ditunjukkan dengan parameter uji: selektifitas, akurasi, presisi, linieritas, batas deteksi dan batas kuantifikasi. Metode identifikasi dan penetapan kadar mitragynine secara KCKT-PDA menunjukkan hasil uji linier pada rentang konsentrasi 1,96 - 6,01 µg/mL dengan koefisien korelasi 0,9996. Batas deteksi metode yaitu 0,14 µg/mL, sedangkan batas kuantifikasi 0,45 µg/mL. Uji akurasi metode adalah 98,88 - 101,44% dan bias 0,27%. Presisi metode dan intermediet secara berturut-turut yaitu adalah 0,67% dan 1,56%. Kandungan mitragynine berkisar antara 6,01 hingga 6,31 mg/g bahan daun kering. Berdasarkan hasil penelitian, dapat disimpulkan bahwa metode yang dikembangkan cepat, sederhana, andal, akurat, dan valid serta memiliki keunggulan dibandingkan metode yang ada dalam hal kesederhanaan persiapan sampel, waktu analisis yang singkat, dan efektivitas biaya dibandingkan dengan GCMS dan LCMS/MS dan dapat diaplikasikan untuk analisis sampel Kratom.

Unduhan

Data unduhan tidak tersedia.

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