Main Article Content
Abstract
mL to 42,300 ng/mL, with the average concentration of isolated DNA was 41,777 ng/mL. For the value of the purity of the isolated DNA whose absorbance was read using a nanophotometer at a wavelength of A260/A280, the results were between 2,301 to 2,384 with the average value of purity being at 2,326. This study concludes that all the extracted samples that showed the results of the DNA analysis produced were included in the good DNA category.
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References
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References
Abinawanto, Sophian, A., Lestari, R., Bowolaksono, A., Efendi, P. S., & Afnan, R. (2019). Analysis of IGF-1 gene in ayam ketawa (Gallus gallus domesticus) with dangdut and slow type vocal characteristics. Biodiversitas, 20(7), 2004–2010. https://doi.org/10.13057/biodiv/d200729
Aminah, A., Ramadini, R., & Naid, T. (2019). Analisis Cemaran DNA Tikus pada Bakso Daging Sapi yang Beredar di Makassar dengan Metode Polymerase Chain Reaction (PCR). Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) (e-Journal), 5(1), 93–100. https://doi.org/10.22487/j24428744.2019.v5.i1.12036
Eppendorf. (2016). Nucleic Acid Photometry. 22331.
Intron. (2020). Patho Gene-spin TM DNA / RNA Extraction Kit. 1–2.
Matlock, B. (2015). Assessment of Nucleic Acid Purity. Technical Bulletin NanoDrop Spectrophotometers, 1–2.
Qiagen®. (2014). DNeasy® Mericon® Food Handbook. 06(February), 18–19.
Rohman, A., Rahayu, W. S., Sudjadi, & Martono, S. (2021). The use of real-time polymerase chain reaction combined with specific-species primer for analysis of dog meat dna in meatball. Indonesian Journal of Chemistry, 21(1), 225–233. https://doi.org/10.22146/ijc.48930
Septiani, T. (2021). Identification of Rat Meatballs in Traditional Market in Area of Jakarta Using Real Time – Pcr. Indonesia Journal of Halal, 3(1), 94–99. https://ejournal2.undip.ac.id/index.php/ijh/article/view/9244
Sophian, A. (2021). Short Communication: Analysis of purity and concentration of extracted DNA on salted fish processed food products. Asian Journal of Natural Product Biochemistry, 19(1), 21–24. https://doi.org/10.13057/biofar/f190104
Sophian, A., Purwaningsih, R., Muindar, Igirisa, E. P. J., & Amirullah, M. L. (2021). Short Communication: Analysis of purity and concentration of DNA extracted from intron patho gene-spin extraction on crab processed food product samples. Asian Journal of Tropical Biotechnology, 18(1), 13–27. https://doi.org/10.13057/biotek/c180103
Sophian, A. (2021). Analysis of the Results of DNA Isolation from Chicken Feather Sampled from the Base of the Young Feathers, the base of the Old Feathers and the Ends of the Feathers. BIOEDUSCIENCE, 5(2). https://doi.org/10.22236/j.bes/526211
Suryawan, G. Y., Suardana, I. W., & Wandia, I. N. (2020). Sensitivity of polymerase chain reaction in the detection of rat meat adulteration of beef meatballs in Indonesia. Veterinary World, 13(5), 905–908. https://doi.org/10.14202/vetworld.2020.905-908
University, E. (2020). EIGC . 005 _ Quantitation with NanoDrop Standard Operating Procedure Staff Review Page : Standard Operating Procedure Approval Page : Manual Prosedure, 1–5.