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Submitted
September 5, 2021
Accepted
November 8, 2021
Published
November 30, 2021
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Abstract

Analysis of the purity and concentration of isolated DNA in the manufacture of standard rat DNA was carried out to see whether the isolation carried out could produce good quality DNA. The purpose of this study is to provide information on the manufacture of raw DNA in species DNA testing where the raw material that has been purchased so far made from synthetic materials can be more economical if using DNA material derived from the meat raw material of the target species. The DNA extraction method used is the column spin method or column centrifuge using the Intron Patho Gene-Spin (Viral DNA/RNA) extraction kit. Analysis method of concentration and purity of isolated DNA was analyzed based on the average value of concentration and purity which was read using a nanophotometer. Based on the results of the research conducted, the results of the isolated DNA concentration values ​​were in the concentration range of 41,250 ng/
mL to 42,300 ng/mL, with the average concentration of isolated DNA was 41,777 ng/mL. For the value of the purity of the isolated DNA whose absorbance was read using a nanophotometer at a wavelength of A260/A280, the results were between 2,301 to 2,384 with the average value of purity being at 2,326. This study concludes that all the extracted samples that showed the results of the DNA analysis produced were included in the good DNA category.

Keywords

DNA Rat Intron Isolation DNA, Tikus, Isolasi, Intron DNA Tikus Isolasi Intron

Article Details

How to Cite
Sophian, A., & Syukur, A. . (2021). Analysis of Purity and Concentration of Isolated DNA in Making Raw DNA of Rat Species: Analysis of Purity and Concentration of Isolated DNA in Making Raw DNA of Rat Species. Eruditio : Indonesia Journal of Food and Drug Safety, 1(2), 1–5. https://doi.org/10.54384/eruditio.v1i2.75
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References

  1. Abinawanto, Sophian, A., Lestari, R., Bowolaksono, A., Efendi, P. S., & Afnan, R. (2019). Analysis of IGF-1 gene in ayam ketawa (Gallus gallus domesticus) with dangdut and slow type vocal characteristics. Biodiversitas, 20(7), 2004–2010. https://doi.org/10.13057/biodiv/d200729
  2. Aminah, A., Ramadini, R., & Naid, T. (2019). Analisis Cemaran DNA Tikus pada Bakso Daging Sapi yang Beredar di Makassar dengan Metode Polymerase Chain Reaction (PCR). Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) (e-Journal), 5(1), 93–100. https://doi.org/10.22487/j24428744.2019.v5.i1.12036
  3. Eppendorf. (2016). Nucleic Acid Photometry. 22331.
  4. Intron. (2020). Patho Gene-spin TM DNA / RNA Extraction Kit. 1–2.
  5. Matlock, B. (2015). Assessment of Nucleic Acid Purity. Technical Bulletin NanoDrop Spectrophotometers, 1–2.
  6. Qiagen®. (2014). DNeasy® Mericon® Food Handbook. 06(February), 18–19.
  7. Rohman, A., Rahayu, W. S., Sudjadi, & Martono, S. (2021). The use of real-time polymerase chain reaction combined with specific-species primer for analysis of dog meat dna in meatball. Indonesian Journal of Chemistry, 21(1), 225–233. https://doi.org/10.22146/ijc.48930
  8. Septiani, T. (2021). Identification of Rat Meatballs in Traditional Market in Area of Jakarta Using Real Time – Pcr. Indonesia Journal of Halal, 3(1), 94–99. https://ejournal2.undip.ac.id/index.php/ijh/article/view/9244
  9. Sophian, A. (2021). Short Communication: Analysis of purity and concentration of extracted DNA on salted fish processed food products. Asian Journal of Natural Product Biochemistry, 19(1), 21–24. https://doi.org/10.13057/biofar/f190104
  10. Sophian, A., Purwaningsih, R., Muindar, Igirisa, E. P. J., & Amirullah, M. L. (2021). Short Communication: Analysis of purity and concentration of DNA extracted from intron patho gene-spin extraction on crab processed food product samples. Asian Journal of Tropical Biotechnology, 18(1), 13–27. https://doi.org/10.13057/biotek/c180103
  11. Sophian, A. (2021). Analysis of the Results of DNA Isolation from Chicken Feather Sampled from the Base of the Young Feathers, the base of the Old Feathers and the Ends of the Feathers. BIOEDUSCIENCE, 5(2). https://doi.org/10.22236/j.bes/526211
  12. Suryawan, G. Y., Suardana, I. W., & Wandia, I. N. (2020). Sensitivity of polymerase chain reaction in the detection of rat meat adulteration of beef meatballs in Indonesia. Veterinary World, 13(5), 905–908. https://doi.org/10.14202/vetworld.2020.905-908
  13. University, E. (2020). EIGC . 005 _ Quantitation with NanoDrop Standard Operating Procedure Staff Review Page : Standard Operating Procedure Approval Page : Manual Prosedure, 1–5.