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Ultra Performance Liquid Chromatography (UPLC) adalah pemisahan analitik yang mempertahankan kepraktisan dan prinsip High Performance Liquid Chromatography (HPLC) dengan meningkatkan kecepatan analisis, sensitivitas, dan resolusi. Selama ini metode yang digunakan untuk identifikasi efedrin dan pseudoefedrin dilakukan menggunakan HPLC sesuai MA PPOMN 07/NA/2010 dari Pusat Pengembangan Pengujian Obat dan Makanan Nasional (PPPOMN). Tujuan penelitian untuk menilai keunggulan metode UPLC dibandingkan dengan HPLC pada identifikasi Efedrin dan Pseudoefedrin sediaan padat. UPLC yang digunakan UPLC Waters dengan detektor PDA pada panjang gelombang 210 nm, kolom Aqquity UPLC® BEH C18 (50 x 2,1 mm) dengan ukuran partikel 1,7 μm, fase gerak larutan natrium lauril sulfat 25 mM dan acetonitril (60:40), laju alir 0,3 ml/menit, volume injeksi 1 μL. Parameter pembanding yang digunakan dalam validasi senyawa efedrin dan pseudoefedrin dengan metode UPLC dan HPLC adalah spesifisitas dan selektivitas. Validasi metode UPLC menunjukkan waktu retensi untuk efedrin dan pseudoefedrin pada menit ke 3,288 dan 3,048 dengan RSD waktu retensi sebesar 0,3% dan luas area sebesar 0,5%. Resolusi antara efedrin dan pseudoefedrin sebesar 1,9. Lama waktu analisis adalah 27 menit pada HPLC dan 6 menit pada UPLC. Berdasarkan hasil penelitian ini metode UPLC dapat digunakan sebagai alternatif untuk identifikasi efedrin dan pseudoefedrin dalam sediaan padat dengan keunggulan lebih cepat, resolusi lebih baik, penggunaan fase gerak, baku pembanding dan sampel lebih sedikit serta beban kerja lebih singkat dibanding HPLC.

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Efedrine pseudoefedrin UPLC Efedrin pseudoefedrin UPLC

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Cara Mengutip
Ichsan, D. S., & Ebtasari , M. . (2023). Identifikasi Efedrin dan Pseudoefedrin Sediaan Padat dengan Metode Ultra Performance Liquid Chromatography dan High Performance Liquid Chromatography. Eruditio : Indonesia Journal of Food and Drug Safety, 3(2), 99–106. https://doi.org/10.54384/eruditio.v3i2.130
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Referensi

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